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以‘丰香’草莓为试材,通过同源克隆法获得FaHY5 cDNA全长序列,运用生物学软件对该序列及其编码的氨基酸序列进行相关生物信息学及结构功能分析,同时采用半定量及荧光定量方法对FaHY5基因在不同组织和果实不同发育阶段的表达模式进行研究。结果表明,FaHY5全长为501 bp,Gen Bank登录号为KP984791,编码166个氨基酸。蛋白质分子量约为18.136 kD,理论等电点为10.05,C末端存在典型的bZIP保守结构域,是一个非分泌型核定位蛋白。序列比对及进化树分析,HY5在进化的过程中具有高度的保守性。表达谱分析,FaHY5在各组织中均有表达,且在花中表达量最高;而在果实发育过程中,FaHY5主要在前期积累。本研究分离鉴定了草莓中HY5(LONG HYPOCOTYL 5)转录因子并探索了其在草莓中的表达模式,为进一步研究草莓FaHY5转录因子的功能和作用机制提供理论基础。
The full-length cDNA of FaHY5 cDNA was obtained by homologous cloning method using ’Fengxiang’ strawberry as test material. Bioinformatics and structural function analysis of this sequence and its encoded amino acid sequence were carried out by using biological software. Semi-quantitative Fluorescence quantitative method was used to study the expression pattern of FaHY5 in different tissues and fruit at different developmental stages. The results showed that FaHY5 was 501 bp in length and GenBank accession number KP984791 encoded 166 amino acids. The molecular weight of protein is about 18.136 kD, the theoretical isoelectric point is 10.05, and there is a typical bZIP conserved domain at the C-terminus. It is a non-secretory nuclear localization protein. Sequence alignment and phylogenetic tree analysis, HY5 in the evolutionary process is highly conservative. Expression profiling showed that FaHY5 was expressed in all tissues and had the highest expression in flowers. FaHY5 mainly accumulated in the early stage of fruit development. In this study, we isolated and identified the HY5 (LONG HYPOCOTYL 5) transcription factor in strawberry and explored its expression pattern in strawberry, providing a theoretical basis for further study on the function and mechanism of strawberry FaHY5 transcription factor.