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目的:对肾母细胞瘤组织WT1、P53基因缺失情况进行检测。方法:30例散发性肾母细胞瘤组织,其中上皮优势型6例,母细胞优势型14例,间质优势型10例,采用Southern印迹分子杂交技术检测肾母细胞瘤组织WT1、P53基因缺失,并分析其与组织类型的关系。结果:2例WT1基因的缺失为间质优势型肾母细胞瘤,1例P53等位基因缺失为间变型肾母细胞瘤。结论:WT1基因缺失可能与间质优势型肾母细胞瘤的发生有关,P53基因缺失可能与间变型肾母细胞瘤的发生有关。
Objective: To detect the deletion of WT1 and P53 genes in nephroblastoma tissue. Methods: Thirty patients with sporadic nephroblastoma were enrolled in this study. Among them, epithelial dominant type was 6, dominant type was 14, and interstitial dominant type was 10. Southern blotting was used to detect the deletion of WT1 and P53 genes in nephroblastoma , And analyze its relationship with the type of organization. Results: Two cases of WT1 gene deletion were interstitial nephroblastoma, and one case of P53 allele deletion was metaphase nephroblastoma. Conclusion: The loss of WT1 gene may be related to the occurrence of interstitial nephroblastoma. The loss of P53 gene may be related to the occurrence of anaplastic nephroblastoma.