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目的对水源水和腹泻病人标本中检测到的17株产H2S致泻大肠埃希菌进行一系列微生物学实验研究,为进一步了解该菌的流行病学特点以及临床诊断提供实验依据。方法自1999年10月～2005年8月,从腹泻病人标本和水源水中分离的产H2S致泻大肠埃希菌,经形态学、培养特性、生化学、血清学等微生物学鉴定,并且对菌株进一步进行了药敏试验和噬菌体裂解试验。结果在腹泻、食物中毒病人标本和水源水中分离到17株产H2S致泻大肠埃希菌,经血清学分型后,其中4株是EPEC O128:K67,ETEC O25:K19和ETEC O16:K15分别为3株,占58.8%。结论产H2S致泻大肠埃希菌与大肠埃希菌有密切的亲缘关系,是变异的致泻大肠埃希菌,在致泻大肠埃菌中占有较高的比例。致泻大肠埃希菌产H2S生化变异提示我们在注意生化反应典型的致病菌分离与鉴定时,还应注意生化变异菌株的检测和鉴定,防止该菌造成新的腹泻病的传播和蔓延。
Objective To investigate a series of microbiological experiments on 17 strains of Escherichia coli producing H2S that were detected in samples of patients with water-source water and diarrhea, and provide experimental evidence for further understanding of the epidemiological characteristics and clinical diagnosis of this bacterium. Methods From October 1999 to August 2005, Escherichia coli producing H2S diarrhea isolated from diarrhea patients and water source water were identified by morphology, culture characteristics, biochemistry, serology and other microorganisms. Further susceptibility testing and phage lysis were carried out. Results 17 strains producing H2S diarrheal Escherichia coli were isolated from diarrhea, food poisoning patients and water sources. After serological classification, 4 strains were EPEC O128: K67, ETEC O25: K19 and ETEC O16: K15, respectively 3 strains, accounting for 58.8%. Conclusion H2S-producing Escherichia coli is closely related to Escherichia coli and is a variant diarrheal Escherichia coli, which accounts for a high proportion of diarrhea-causing Escherichia coli. Diarrheogenic Escherichia coli biochemical H2S biochemical variation prompted us to pay attention to the biochemical reactions of typical pathogenic bacteria isolation and identification, but also pay attention to the detection of biochemical variants of the strain and identification, to prevent the bacteria caused by the spread and spread of new diarrhea.