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目的分析100例大肠癌患者VEGF、EGFR、Ki-67及P53基因表达与肿瘤浸润转移的相关性。方法选取100例行肠电子内镜活检组织,病理确诊为大肠癌行手术治疗的患者为研究对象,根据患者大肠癌Dukes分期分成两组,其中A组为Dukes分期D期57例,B组为Dukes分期A+B+C期43例,随机从B组中选取15例,取手术标本切缘正常大肠黏膜做对照,为C组,免疫组化Envision法对组织中VEGF、EGFR、Ki-67、P53进行检测。观察三组标本相关指标表达差异。结果 A组VEGF、EGFR、Ki-67、P53表达总阳性率为82.5%、80.7%、84.2%、82.5%,B组表达总阳性率为76.7%、72.1%、69.8%、67.4%,C组组表达总阳性率为13.3%、13.3%、6.7%、6.7%,三组VEGF、EGFR、Ki-67、P53表达率等级资料分析差异有统计学意义(P<0.05)。结论 VEGF、EGFR、Ki-67及P53基因表达与大肠癌的浸润转移有着密切的关系,检测VEGF、EGFR、Ki-67及P53的表达情况对判断大肠癌预后及指导治疗有一定的参考意义。
Objective To analyze the correlation between the expression of VEGF, EGFR, Ki-67 and P53 and the infiltration and metastasis of tumor in 100 cases of colorectal cancer. Methods 100 cases of intestinal electronic endoscopic biopsy were selected and pathologically diagnosed as colorectal cancer patients undergoing surgical treatment were divided into two groups according to the Dukes stage of colorectal cancer, in which group A was Duke stage D 57 cases, group B was Dukes stage A + B + C 43 cases, 15 cases were randomly selected from group B, and the control group was normal colorectal mucosa as the control group, C group, immunohistochemical Envision method of VEGF, EGFR, Ki-67 , P53 for testing. The difference between the three groups was observed. Results The positive rates of VEGF, EGFR, Ki-67 and P53 in group A were 82.5%, 80.7%, 84.2% and 82.5%, respectively, and the positive rates in group B were 76.7%, 72.1%, 69.8% and 67.4% The positive rate of expression of VEGF, EGFR, Ki-67 and P53 in the three groups was statistically significant (P <0.05). The positive rates of the three groups were 13.3%, 13.3%, 6.7% and 6.7% respectively. Conclusion The expressions of VEGF, EGFR, Ki-67 and P53 are closely related to the invasion and metastasis of colorectal cancer. Detecting the expressions of VEGF, EGFR, Ki-67 and P53 may be useful for judging the prognosis and guiding therapy of colorectal cancer.