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目的介绍微型渗透压泵进行耳蜗灌注的方法，达到耳蜗慢性给药。方法将耳蜗埋置管置于５只灰鼠耳蜗底回鼓阶中，再将渗透压泵埋置于动物背部，利用泵内、外渗透压之差将泵内液体缓慢挤压释放。观察耳蜗灌流Ｈａｎｋ液（３耳）和Ｌｂｕｔｈｉｏｎｉｎｅ［Ｓ，Ｒ］ｓｕｌｆｏｘｉｍｉｎｅ（ＢＳＯ）液（２耳）对下丘电位和耳蜗毛细胞的影响。结果应用渗透压泵耳蜗灌流Ｈａｎｋ液后，下丘电位阈值维持正常，耳蜗毛细胞无损伤；灌流ＢＳＯ液１４天后，所测频率（１、２、４和８ｋＨｚ）的下丘电位阈值平均升高２８ｄＢ，内耳毛细胞平均缺失３９．３％，缺失分布于耳蜗各回，表明渗透压泵工作良好。结论应用渗透压泵进行耳蜗灌流是一种慢性耳蜗给药的有效方法，灌流Ｈａｎｋ液不会对耳蜗产生明显影响。
Objective To introduce the method of mini-osmotic pump for cochlear perfusion to achieve chronic administration of cochlea. Methods The cochlear implant tube was placed in the bottom of the cochlea of 5 squirrels. The osmotic pressure pump was embedded in the back of the animal. The difference of osmotic pressure inside and outside the pump was used to slowly squeeze the liquid in the pump. The effects of Hank’s solution (3 ears) and Lbuthionine [S, R] sulfoximine (BSO) solution (2 ears) on the hypothalamic potential and cochlear hair cells were observed. Results Hypothalamic threshold was maintained and cochlear hair cells were not damaged after Hank’s solution was infiltrated by cochlear implants. After 14 days perfusion of BSO solution, the threshold of hypothalamus increased at average frequency (1, 2, 4 and 8 kHz) 28dB, the average loss of hair cells in the inner ear 39.3%, missing distribution in the cochlear back, indicating that the osmotic pump work well. Conclusion Cochlear perfusion is an effective method of chronic cochlear administration, and perfusion of Hank’s solution does not affect the cochlea significantly.