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目的探讨献血者酶联免疫吸附试验法(ELISA法)检测乙型肝炎表面抗原(HBs Ag)为灰区标本采用中和试验确认情况。方法 260例ELISA法检测HBs Ag灰区标本(0.70.05);C组阳性率显著高于A组和B组,差异具有统计学意义(P<0.05);双试剂灰区确认阳性率高于单试剂灰区,差异有统计学意义(P<0.05)。结论 ELISA法检测HBs Ag存在一定程度的漏检,采用中和试验确认可减少HBs Ag的漏检及提高血液安全,避免血液资源的浪费。
Objective To investigate the detection of hepatitis B surface antigen (HBsAg) by gray scale in blood donors by enzyme-linked immunosorbent assay (ELISA). Methods 260 cases of HBsAg gray zone samples (0.7 0.05). The positive rate of C group was significantly higher than that of A group and B group Group, the difference was statistically significant (P <0.05). The positive rate of double reagent gray zone was higher than that of single reagent gray zone, the difference was statistically significant (P <0.05). Conclusion The detection of HBs Ag by ELISA shows that there is a certain degree of misdetection. The neutralization test can reduce the missed detection of HBsAg and improve the blood safety and avoid the waste of blood resources.