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目的：建立经济实用的胎儿宫内窘迫动物模型。方法：利用35只20天胎龄的胎鼠，钳夹进入每个胎盘的分支血管，共15分钟，然后松开血管夹，恢复血流，对胎鼠断头处死后进行显微镜检查和测定脑细胞内外钙、钠、钾含量。结果：再灌注15分钟后处死而进行显微镜检查的全部25只胎鼠脑组织均显示结构紊乱，间质和星形细胞水肿，以及神经细胞变性，6／25只（24％）出现神经细胞坏死，7／25只（28％）脑出血。另10只胎鼠在再灌注30分钟后处死进行Fura－2AM法检测，结果显示脑细胞内出现钙超载。结论：此模型可以较好地用于胎儿宫内窘迫脑损害的进一步研究。
Objective: To establish an economical and practical animal model of fetal distress. METHODS: Twenty-five (20) gestational day fetal mice were grafted into the branch vessels of each placenta for 15 minutes, and then the vessel clips were released and the blood flow was restored. Microscopes and brains were performed on the fetal rats after decapitation Intracellular calcium, sodium, potassium content. RESULTS: Brain tissue from all 25 fetal rats sacrificed 15 minutes after reperfusion and underwent microscopic examination revealed structural disorders, interstitial and astrocytic edema, and degeneration of nerve cells. Neuronal cell necrosis occurred in 6 of 25 (24%) , 7/25 (28%) cerebral hemorrhage. Another 10 fetuses were sacrificed 30 minutes after reperfusion, and Fura-2AM assay was performed. The results showed that calcium overload occurred in brain cells. Conclusion: This model can be used for further study of fetal brain damage in fetal distress.