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目的探讨磁性纳米四氧化三铁颗粒(Fe3O4-MNPs)逆转人卵巢癌顺铂耐药细胞株(SKOV3/DDP)耐药性的可能性。方法将SKOV3/DDP细胞分为对照组、顺铂(DDP)组、Fe3O4-MNPs组和DDP+Fe3O4-MNPs组,体外培养后MTT法测定细胞生长半数抑制浓度(IC50),流式细胞术测定细胞凋亡及电感耦合等离子体原子发射光谱(ICP)法测定细胞内DDP含量。结果DDP组IC50为39.31μM,DDP+Fe3O4-MNPs组为17.4μM(P<0.05)。DDP+Fe3O4-MNPs组细胞凋亡率较DDP组明显提高(P<0.05)。DDP+Fe3O4-MNPs组细胞内顺铂含量明显高于DDP组(P<0.05)。结论Fe3O4-MNPs可与化疗药物顺铂协同,逆转卵巢癌细胞对DDP的耐受性。其作用可能是通过提高细胞内药物聚集量实现的。
Objective To investigate the possibility of reversing drug resistance of human ovarian cancer cisplatin-resistant cell line (SKOV3 / DDP) with magnetic nano-Fe3O4-MNPs. Methods The SKOV3 / DDP cells were divided into control group, DDP group, Fe3O4-MNPs group and DDP + Fe3O4-MNPs group. The cell growth inhibitory concentration (IC50) was determined by MTT assay in vitro. Flow cytometry Cell apoptosis and inductively coupled plasma atomic emission spectrometry (ICP) were used to determine intracellular DDP content. Results IC50 was 39.31μM in DDP group and 17.4μM in DDP + Fe3O4-MNPs group (P <0.05). The apoptosis rate of DDP + Fe3O4-MNPs group was significantly higher than that of DDP group (P <0.05). The content of cisplatin in DDP + Fe3O4-MNPs group was significantly higher than that in DDP group (P <0.05). Conclusion Fe3O4-MNPs can cooperate with cisplatin in chemotherapy and reverse the tolerance of ovarian cancer cells to DDP. Its role may be achieved by increasing the amount of intracellular drug accumulation.