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细胞色素P450酶系对杀虫剂的代谢作用是大多数昆虫产生抗药性的主要机制,沉默P450基因能否对烟粉虱抗药性产生影响需要进一步研究。本论文将含Q型烟粉虱细胞色素P450基因CYP6CM1 RNAi表达框架的转基因烟草饲喂烟粉虱,荧光定量PCR检测发现烟粉虱取食转基因烟草后体内CYP6CM1基因表达量显著下降,仅为取食非转基因烟草烟粉虱的19%~38%;通过琼脂保湿浸叶法和单株活体法研究取食转基因烟草烟粉虱对阿维·吡虫啉抗药性的影响,结果发现在接触1 g/L阿维·吡虫啉24~72 h后,取食转基因烟草的烟粉虱死亡率分别比取食非转基因烟草的烟粉虱死亡率显著升高20%~30%和15%~20%。本研究结果表明,饲喂烟粉虱转CYP6CM1 RNAi基因烟草可以显著降低烟粉虱细胞色素P450 CYP6CM1基因的表达,并降低烟粉虱对烟碱类杀虫剂阿维·吡虫啉的抗药性,显著提高了杀虫剂防治效果。
Cytochrome P450 enzymes on the metabolism of insecticides is the main mechanism of the resistance of most insects, silencing the P450 gene can affect the resistance of Bemisia tabaci insects need further study. In the present study, B. tabaci were fed on transgenic tobacco containing the CYP6CM1 RNAi expression frame of cytochrome P450 gene of Q Bemisia tabaci. Fluorescent quantitative PCR showed that CYP6CM1 gene expression decreased significantly in B. tabaci fed on transgenic tobacco, Non-transgenic tobacco whitefly Bemisia tabaci 19% to 38%; by agar moisturizing leaf immersion method and single living method to study the impact of feeding on transgenic tobacco whiteflies on Avian imidacloprid resistance and found that exposure to 1 g / L. The mortality of Bemisia tabaci feeding on transgenic tobacco was 20% -30% and 15% -20% more than that of non-transgenic tobacco on 24-26 hours. The results of this study showed that feeding B. tabaci transgenic tobacco with CYP6CM1 RNAi could significantly reduce the gene expression of cytochrome P450 CYP6CM1 and reduce the resistance of B. tabaci to the nicotine insecticide, Improve the effectiveness of pesticide control.