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在玉米小孢子培养再生植株的生根培养基添加1.0mg/LIBA、0.05mg/LNAA和1mg/LMET,能明显抑制株高,促进发根,再生植株生长粗壮,根系发达,移栽后成活率高。移栽前要开瓶炼苗,时间以24-48h为宜。移栽时先用蛭石和珍珠岩作为基质,培育5~7d后,然后再移入耕作土加1/3充分腐熟鸡粪的基质中,再生植株的成活率可达90%以上,成活的再生植株叶色浓绿,根系发达,长势良好。通过测量气孔保卫细胞长度来评估再生植株的染色体倍性是一种简单可行的方法。
The addition of 1.0mg / LIBA, 0.05mg / L NAA and 1mg / LMET to the rooting medium of the regenerated plants could significantly inhibit the plant height and promote hairy roots. The regenerated plants grew well, the root system developed and the survival rate after transplanting was high . Before transplanting to open bottle refining, time to 24-48h appropriate. In the transplanting, vermiculite and perlite were used as the substrate. After cultivating for 5 ~ 7 days, they were transferred into the soil of cultivated soil plus 1/3 full-maturity chicken manure. The survival rate of the regenerated plants could reach over 90%, and the viable regenerated plants Leaf color dark green, well-developed, growing well. Evaluating the chromosome ploidy of regenerated plants by measuring stomatal guard cell length is a simple and viable method.