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α-Glucosidase and lipase inhibitors play important roles in the treatment of hyperglycaemia and dyslipidemia. To identify novel naturally occurring inhibitors, a bioactivity-guided phytochemical research was performed on the pu-erh tea. One new flavanol, named(–)-epicatechin-3-O-(Z)-coumarate(1), and 16 known analogs(2–17) were isolated from the aqueous extract of the pu-erh tea. Their structures were determined by spectroscopic and chemical methods. Furthermore, the water extract of pu-erh tea and its fractions exhibited inhibitory activities against α-glucosidases and lipases in vitro;compound 15 showed moderate inhibitory effect against sucrase with an IC_(50) value of 32.5 μmol/L and significant inhibitory effect against maltase with an IC_(50) value of 1.3 μmol/L. Compounds 8, 10, 11 and15 displayed moderate activity against a lipase with IC_(50) values of 16.0, 13.6, 19.8, and 13.3 μmol/L,respectively.
α-Glucosidase and lipase inhibitors play important roles in the treatment of hyperglycaemia and dyslipidemia. To identify novel naturally occurring inhibitors, a bioactivity-guided phytochemical research was performed on pu-erh tea. One new flavanol, named (-) - epicatechin- Their structures were determined by spectroscopic and chemical methods. Furthermore, the water extract (2-17) was isolated from the aqueous extract of the pu-erh tea of pu-erh tea and its fractions check inhibitory activities against a-glucosidases and lipases in vitro; compound 15 (10) 50) value of 1.3 μmol / L. Compounds 8, 10, 11 and 15 displayed moderate activity against a lipase with IC 50 values of 16.0, 13.6, 19.8, and 13.3 μmol / L, respectively.