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目的:探讨肺癌患者外周血CD4+CD25+调节性T细胞(Treg)的比例变化及其在肿瘤发生发展中的作用。方法:应用流式细胞术检测78例肺癌患者和30例健康者CD4+CD25+Treg比例,分析其与肺癌的临床分期、病理类型、组织学分化程度及手术的关系。结果:肺癌患者外周血CD4+CD25+Treg比例与健康对照组比较,差异有统计学意义,t=2.316,P=0.04。Ⅲ、Ⅳ期肺癌患者Treg比例显著高于Ⅰ+Ⅱ期患者,t值分别为2.205和2.207,P值均为0.04。鳞癌、腺癌、小细胞肺癌高、中和低分化肺癌患者Treg比例显著高于对照组,P<0.05。手术后的肺癌患者Treg比例为(14.38±3.82)%,显著低于手术前的(20.16±5.24)%,t=1.823,P=0.05。结论:肺癌患者外周血CD4+CD25+Treg水平明显升高,且与肺癌的进展密切相关,越晚期水平越高;手术后肺癌患者外周血CD4+CD25+Treg水平明显下调。
Objective: To investigate the proportion of CD4 + CD25 + regulatory T cells (Tregs) in peripheral blood of patients with lung cancer and its role in tumorigenesis. Methods: The proportion of CD4 + CD25 + Treg in 78 lung cancer patients and 30 healthy controls was detected by flow cytometry. The relationship between CD4 + CD25 + Treg and clinical stage, pathological type, histological differentiation and operation of lung cancer was analyzed. Results: The percentage of CD4 + CD25 + Treg in peripheral blood of patients with lung cancer was significantly lower than that of healthy controls (t = 2.316, P = 0.04). The proportion of Tregs in stage Ⅲ and Ⅳ lung cancer patients was significantly higher than that in stage Ⅰ + Ⅱ patients, with t values of 2.205 and 2.207, respectively, with P values of 0.04. The proportion of Treg in squamous cell carcinoma, adenocarcinoma, small cell lung cancer patients with high, moderate and poorly differentiated lung cancer was significantly higher than that in the control group (P <0.05). The percentage of Treg in postoperative lung cancer patients was (14.38 ± 3.82)%, significantly lower than that before operation (20.16 ± 5.24)%, t = 1.823, P = 0.05. Conclusion: The levels of CD4 + CD25 + Treg in peripheral blood of patients with lung cancer are significantly increased, and are closely related to the progress of lung cancer. The higher the level is, the lower the level of CD4 + CD25 + Treg in peripheral blood of patients with lung cancer.