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本文介绍了一种结合原位杂交法,利用胰酶/EDTA诱导染色体显带的简易且能重复的方法。由同步化或非同步化的正常人外周血淋巴细胞和经培养的单层成纤维细胞制备染色体标本。用~3H标记的DNA探针杂交和放射自显影测定以后,将染色体标本放在
This article presents a simple and reproducible method for the visualization of chromosomes using in situ hybridization with trypsin / EDTA. Chromosome specimens were prepared from synchronized or unsynchronized normal human peripheral blood lymphocytes and cultured monolayers of fibroblasts. After hybridization with ~ 3H-labeled DNA probe and autoradiography, chromosomal specimens were placed in