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自从在脑内发现阿片肽以来,有人曾在脑内寻找是否存在着内源性的抗阿片物质。目前知道,促黑激素释放抑制因子、ACTH、β-内啡肽片断及八肽胆囊收缩素等均具有对抗阿片物质的作用。但实际上,这些肽都不是以抗阿片物质的形式真正从脑组织中分离提取出来。本文作者用100只牛脑(40kg),先经丙酮提取得到7.5kg干粉。然后,经分子筛、离子交换以及高压液相层析等步骤多次分离纯化,最终得到两个活性峰A和B。活性峰B已纯化为单一肽,并经氨基酸成分分析与[缬~5]-血管紧张肽Ⅰ(十肽)一致。在高压液相中该肽与合成的[缬~5]-血管紧张肽Ⅰ具有相同的保留时间(Rt)。A峰尚未完全纯化,但在四种不同流洗系统高压液相上的Rt与合成的[缬~5]-血管紧张肽Ⅱ
Ever since opioid peptides were found in the brain, there have been searches in the brain for the presence of endogenous anti-opioids. It is known that melatonin release inhibitors, ACTH, β-endorphin fragments, and octapeptide cholecystokinin all have opioid-fighting effects. But in fact, none of these peptides are actually extracted from brain tissue in the form of anti-opioids. The authors used 100 bovine brain (40kg), first acetone extract to get 7.5kg dry powder. Then, the molecular sieves, ion exchange and high pressure liquid chromatography and other steps to separate and purify several times, and finally get two active peaks A and B. Active Peak B has been purified as a single peptide and analyzed by amino acid composition consistent with [Val-5] - Angiotensin I (Decapeptide). The peptide has the same retention time (Rt) as the synthesized [val-5] -tiptie I in the high pressure liquid phase. A peak has not yet been completely purified, but in four different flow system on the high-pressure liquid Rt and synthetic [Valerian ~ 5] - angiotensin II