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人大颗粒淋巴细胞(LGL)具有NK细胞活性,表面有T细胞和髓单核细胞的标志,可有类似T细胞的作用,如产生IL 2和α、γ干扰素等.本文报导人LGL能产生髓单核细胞因子IL 1.作者取健康人外周血淋巴细胞(经贴壁和尼龙柱法除去粘附细胞)用不连续Percoll密度梯度离心法获取不同组分的细胞,然后测定NK细胞活性和IL 1的产量,组分1和组分2分别含有30～40%和5～10%的单核细胞,其余为LGL;组分3主要是LGL(80～85%),大多细胞有NK细胞标志(67%OKM1~+,62%OKT10+,75%B
Human granulocyte lymphocyte (LGL) has NK cell activity, the surface of T cells and myelomonocytic markers, may have similar T-cell effects, such as the production of IL 2 and α, γ interferon, etc. It is reported herein that human LGL can produce Myelomonocytokine IL 1. The authors take healthy peripheral blood lymphocytes (adherent and nylon column adherent cells removed) by discontinuous Percoll density gradient centrifugation to obtain different components of the cells, and then determine the activity of NK cells and IL 1 production, component 1 and component 2 contain 30-40% and 5-10% monocytes, respectively, with the remainder LGL; fraction 3 mainly LGL (80-85%), most cells with NK cells Logo (67% OKM1 ~ +, 62% OKT10 +, 75% B