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目的:研究姜黄素水解物对K562/A02细胞多药耐药的逆转作用,初步探讨其逆转机制。方法:MTT法检测姜黄素水解物处理后K562/A02细胞对常用化疗药物敏感性的变化;免疫组织化学法检测姜黄素水解物处理后K562/A02细胞与其亲本K562细胞膜P-gp的表达;流式细胞术检测K562和K562/A02细胞内柔红霉素(DNR)的平均荧光强度(mean fluo-rescene intendity,MFI);RT-PCR法检测K562/A02细胞mdr1 mRNA。结果:用2.5 mg.L-1姜黄素水解物处理后K562/A02细胞对常用化疗药物敏感性提高;姜黄素水解物能降低K562/A02细胞膜P-gp的表达(P<0.05)。K562/A02细胞内DNR的MFI低于K562细胞(P<0.01),姜黄素水解物能增加K562/A02细胞内DNR的MFI(P<0.05);姜黄素水解物处理后K562/A02细胞内mdr1 mRNA下降。结论:姜黄素水解物具有体外逆转K562/A02细胞多药耐药的作用,且降低K562/A02细胞膜P-gp的表达降低化疗药物外排增强化疗药物在细胞内的潴留可能是其逆转作用的机制之一。
Objective: To study the reversal effect of curcumin hydrolysate on multidrug resistance in K562/A02 cells, and to explore its reversal mechanism. METHODS: The sensitivity of K562/A02 cells to common chemotherapeutic drugs was detected by MTT assay. The expression of P-gp in K562/A02 cells and their parental K562 cells was detected by immunohistochemistry after flow of curcumin hydrolysate. The mean fluorescence intensity of daunorubicin (DNR) in K562 and K562/A02 cells was detected by using cell cytometry. The mdr1 mRNA in K562/A02 cells was detected by RT-PCR. RESULTS: The sensitivity of K562/A02 cells treated with 2.5 mg.L-1 curcumin hydrolysate was higher than that of commonly used chemotherapy drugs; Curcumin hydrolyzate could reduce the expression of P-gp in K562/A02 cell membrane (P<0.05). The MFI of DNR in K562/A02 cells was lower than that of K562 cells (P<0.01). The curcumin hydrolysate increased the MFI of DNR in K562/A02 cells (P<0.05); mdr1 in K562/A02 cells after curcumin hydrolysate treatment. mRNA decreased. CONCLUSION: The curcumin hydrolysate has the effect of reversing multidrug resistance in K562/A02 cells in vitro, and decreases the expression of P-gp in K562/A02 cell membrane. It is possible that the curcumin hydrolysate can reverse the effect of chemotherapeutic drug efflux and intracellular retention of chemotherapeutic drugs. One of the mechanisms.