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目的 研究穿龙薯蓣总皂苷中水溶性甾体皂苷 ,寻找新的活性化合物。方法 用硅胶柱色谱、薄层色谱及高效液相色谱等进行分离 ,通过酸水解、理化常数和波谱学分析 (IR ,NMR ,MS ,HMQC ,HMBC)鉴定化合物结构。结果 从穿龙薯蓣总皂苷中分得 2个甾体皂苷 (1个水难溶性皂苷和 1个水溶性皂苷 ) ,其化学结构分别鉴定为薯蓣皂苷元 3 O {α L 鼠李糖 (1→2 ) [β D 葡萄糖 (1→ 3) ]} β D 葡萄糖皂苷 (I) ,薯蓣皂苷元 3 O [α L 鼠李糖 (1→ 3) α L 鼠李糖 (1→ 4 ) α L 鼠李糖 (1→ 4 ) ] β D 葡萄糖皂苷 (II)。结论 II为首次从穿龙薯蓣中分离得到的新化合物 ,命名为穿龙薯蓣皂苷Dc。
Objective To study the water-soluble steroidal saponins in the total saponins of Dioscorea nipponica and find new active compounds. Methods The compounds were isolated by silica gel column chromatography, thin layer chromatography and high performance liquid chromatography. The structures of the compounds were identified by acid hydrolysis, physicochemical constants and spectroscopic analysis (IR, NMR, MS, HMQC, HMBC). Results Two steroidal saponins (one water-insoluble saponin and one water-soluble saponin) were obtained from the total saponins of Dioscorea nipponica, and their chemical structures were identified as diosgenin 3 O {α L rhamnose (1→ 2) [β D Glucose (1→3)]} β D Glucosavosin (I), Diosgenin 3 O [α L Rhamnose (1→ 3) α L Rhamnose (1→ 4) α L Rats Liposaccharides (1→4) ] β D Glucososaponin (II). Conclusion II is the first new compound isolated from Dioscorea nipponica and named as Dioscorea dioxin Dc.