论文部分内容阅读
首次建立人精液穿透精子密度直接计数方法，它是一种反映精子穿透女性宫颈粘液（ＣＭ）的定量分析。本法精密度高，作者对穿透精子密度为５５．０×１０９／Ｌ，２７．０×１０９／Ｌ的精液进行重复试验，ＣＶ分别为３．９６％和５．８１％，平均４．８８％。准确性好，通过对精子灭活样本试验，结果直接计数未见精子。本实验与精子尾部低渗肿胀试验（ＨＯＳ）、精液成活精子数（伊红法）和Ⅲ级活力精子数（ＷＨＯ精子活力四级分类法）皆呈正相关，ｒ分别为０．７４５、０．７６０、０．８５０，Ｐ值均＜０．００１；与精液精子密度不相关，ｒ＝０．２５５，Ｐ＞０．０５。正常男子穿透精子密度≥１１．０×１０９／Ｌ。
For the first time, a direct method of counting sperm penetration through human sperm was established, which is a quantitative analysis of sperm penetration into female cervical mucus (CM). This method has high precision. The author repeated the test on sperm penetrating sperm at a density of 55.0 × 109 / L and 27.0 × 109 / L, with CVs of 3.96% and 5.81%, respectively, with an average of 4. 88%. Accuracy, through the sperm inactivation of the sample test, the results of direct counting of no sperm. There was a positive correlation between this experiment and HOS, sperm motility (Ⅲ) and Ⅲ vitality sperm count (WHO sperm motility quadruple classification), r = 0.745,0. 760,0.850, P <0.001; no correlation with semen sperm density, r = 0.255, P> 0.05. Normal men penetrate sperm density ≥ 11.0 × 109 / L.