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用ＲＴ－ＰＣＲ方法从北京株丙型肝炎病毒中扩增出ＮＳ３区基因片段，该片段经ｐＧＥＸ－Ｔ载体克隆到大肠杆菌ＤＨ５α菌株中．经自动序列分析仪测出ＮＳ３基因的７２８ｂｐ长的核酸序列．发现在该片段中第３１位核苷酸发生Ａ→Ｔ突变，产生一个终止突变．这表明，丙型肝炎病毒北京株存在一定的变异，产生缺陷型病毒，这类缺陷型病毒的出现可能是丙型肝炎病毒持续性感染的原因之一．
NS3 region gene was amplified from Beijing strain of hepatitis C virus by RT-PCR. The fragment was cloned into E. coli DH5α strain by pGEX-T vector. The 728 bp long nucleic acid sequence of NS3 gene was detected by automatic sequence analyzer. The A → T mutation was found at nucleotide 31 in this fragment, resulting in a stop mutation. This shows that the hepatitis C virus Beijing strain there are some variations, resulting in defective virus, the emergence of such defective virus may be one of the causes of persistent hepatitis C virus infection.