论文部分内容阅读
以生物测定及羧酸酯酶活性测定的方法,检测了上海地区菜缢管蚜对有机磷的抗药性。测定结果表明,金山群体对乐果、杀螟松和马拉硫磷的抗药性分别是梅陇的5.7、4.0和1.8倍。金山群体的羧酸酯酶活性高于梅陇。说明其抗性与羧酸酯酶活性增高有关。单个蚜虫的羧酸酯酶活性测定是检测抗性及抗性个体在群体内分布的一种既迅速又精确的方法。如把蚜虫的羧酸酯酶活性分成3类:低羧酸酯酶活性(OD<0.3)、中等羧酸酯酶活性(OD:0.3—0.5)和高羧酸酯酶活性(OD>0.6),则金山与梅陇种群低羧酸酯酶活性个体分别占8％、30％;中等活性个体分别占85％、68％;高活性个体分别占7％、2％。
The bioassay and carboxylesterase activity assay were used to detect the resistance of Phylloxerae aphid to organophosphate in Shanghai. The results showed that the resistance of Jinshan population to dimethoate, fenitrothion and malathion were 5.7, 4.0 and 1.8 times that of Meilong, respectively. Jinshan group carboxylesterase activity higher than Meilong. Indicating that its resistance and increased activity of carboxylesterase. The carboxylesterase activity assay of a single aphid is a rapid and accurate method of detecting the distribution of resistant and resistant individuals within a population. For example, the carboxylesterase activities of aphids were classified into three groups: low carboxylesterase activity (OD <0.3), moderate carboxylesterase activity (OD: 0.3-0.5) and high carboxylesterase activity (OD> 0.6) , Individuals of Jinshan and Meilong population with low carboxylesterase activity accounted for 8% and 30%, respectively; medium-activity individuals accounted for 85% and 68% respectively; and high-activity individuals accounted for 7% and 2% respectively.