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在微波法制备的纳米银上获得了大肠杆菌的表面增强拉曼光谱,大肠杆菌在650、952、1125、1242、1320、1372、1459cm-1有明显的拉曼振动峰。其中650cm-1处的振动峰最强,1124、1320、1372和1459cm-1处的四个振动峰较弱,952、1242cm-1处的二个振动峰强度居中。位于650cm-1附近的拉曼峰源自于酪氨酸、鸟嘌呤的振动,952cm-1属于缩氨酸基团C=C的伸缩振动,1124cm-1是蛋白质的C-N和C-C伸缩振动引起的,1242cm-1则归属于是酰胺III蛋白的振动,1320cm-1是蛋白质的CH变形振动,1372cm-1是由酪氨酸振动引起的,1459cm-1则是蛋白质的CH2变形振动。
Surface enhanced Raman spectra of Escherichia coli was obtained on the prepared nanosilver by microwave method. Escherichia coli showed significant Raman peak at 650,952,1125,1242,1320,1372,1459cm-1. Among them, the vibrational peak at 650cm-1 is strongest, the four vibrational peaks at 1124, 1320, 1372 and 1459cm-1 are weak, and the two vibrational peaks at 952 and 1242cm-1 are centered. The Raman peak located at 650 cm -1 originates from the vibration of tyrosine and guanine, 952 cm -1 belongs to the stretching vibration of the peptide group C = C, and 1124 cm -1 is caused by CN and CC stretching vibration of the protein , 1242cm-1 belongs to the vibration of the amide III protein, 1320cm-1 is the CH vibration of the protein, 1372cm-1 is caused by tyrosine vibration, and 1459cm-1 is the protein’s CH2 vibration.