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明确血管紧张素Ｉ转换酶（ＡＣＥ）基因插入／缺失（Ｉ／Ｄ）多态位点与非胰岛素依赖型糖尿病（ＮＩＤＤＭ）及其肾脏合并症发病的关系。方法以ＡＣＥ基因内含子１６的一个２８７ｂｐ的Ａｌｕ顺序Ｉ／Ｄ型为多态标志，用聚合酶链反应（ＰＣＲ）扩增基因片段，１２％非变性聚丙烯酰胺凝胶电泳检测ＰＣＲ产物。结果（１）１２３例ＮＩＤＤＭ与１１０例正常人对照组之间基因频率差异无显著意义；（２）ＮＩＤＤＭ合并肾病与未合并肾病亚组间基因型频率和等位基因频率差异无显著意义，以上均以等位基因Ｉ占优势；（３）ＮＩＤＤＭ发病初期出现的肾病和发病５年以上仍未合并肾病的亚组比较，等位基因Ｄ明显占优势（０．７５），ＤＤ型及ＤＩ型有增多趋势。结论ＡＣＥ基因Ｉ／Ｄ多态是早发ＮＩＤＤＭ肾病的易患因素
To clarify the relationship between angiotensin I converting enzyme (ACE) gene insertion / deletion (I / D) polymorphism and non-insulin dependent diabetes mellitus (NIDDM) and its incidence of renal complications. Methods A 287bp Alu I / D polymorphism of ACE gene intron 16 was amplified by polymerase chain reaction (PCR). The PCR products were detected by 12% non-denaturing polyacrylamide gel electrophoresis. Results (1) There was no significant difference in the frequencies of genes between 123 cases of NIDDM and 110 cases of normal controls. (2) There was no significant difference in the frequencies of genotypes and alleles among NIDDM patients with and without renal nephropathy Allele D predominates (P <0.05); (3) allele D predominates in the group of nephropathy in the early stage of NIDDM onset and in the subgroup of more than 5 years without nephropathy There is an increasing trend. Conclusion ACE gene I / D polymorphism is a predisposing factor for early-onset NIDDM nephropathy