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目的 :研究嘌呤霉素肾病大鼠肾小球足细胞相关分子在病变过程中的表达与分布的关系及变化。 方法 :4 2只SD大鼠随机分为实验组和对照组 ,建立嘌呤霉素肾病模型 ,并在 2 4h、36h、2天、5天、1 0天、1 5天、2 0天七个不同的时间点 ,应用免疫荧光双标记的方法 ,通过激光共聚焦显微镜对足细胞相关分子nephrin、podocin ,α actinin 4以及CD2AP两两之间的分布关系及变化进行研究。 结果 :正常大鼠肾小球中CD2AP与nephrin及podocin的染色沿肾小球血管襻有部分重叠 ,而两者的分布模式略有不同 ,但这种共定位关系不随病变的发展而变化 ;CD2AP与α actinin 4的分布也有部分重叠关系 ,且随着病变进展 ,其荧光重叠程度增加 ;α actinin 4与nephrin及podocin在正常时有极小部分重叠 ,随着病程变化 ,两者的分布模式与分布量的变化均不同步。 结论 :足细胞相关分子nephrin、podocin及CD2AP之间以功能复合体的形式相互联系并参与蛋白尿的发生发展 ,而足细胞相关分子的分子行为可能在蛋白尿的发生中起重要作用
OBJECTIVE: To study the relationship between the expression of glomerular podocyte-related molecules and the distribution of puromycin nephropathy rats in the course of the pathological changes. Methods: Forty-two Sprague-Dawley rats were randomly divided into experimental group and control group. The model of puromycin nephropathy was established. At 4h, 36h, 2d, 5d, 10d, 15d and 20d, At different time points, the distribution relationship and changes of podocin, α actinin 4 and CD2AP between podocin and podocin were studied by laser scanning confocal microscopy. Results: The staining of CD2AP, nephrin and podocin in glomeruli of normal rats partially overlap with the glomerular loop, but their distribution pattern is slightly different. However, the colocalization does not change with the development of the lesion. CD2AP And α actinin 4 also partially overlapped, and the degree of fluorescence overlap increased with the progression of the disease. Α actinin 4 overlap with nephrin and podocin in a very small part of the normal, with the course of disease, the distribution pattern of the two Changes in the amount of distribution are not synchronized. CONCLUSION: The podocyte-related molecules nephrin, podocin and CD2AP are related to each other in the form of functional complexes and are involved in the development of proteinuria. The molecular behavior of podocyte-related molecules may play an important role in the pathogenesis of proteinuria