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为研究fas基因转导对食管癌细胞的体外抑制作用 ,构建fas基因真核表达载体fas pBK并将其转导入食管癌细胞EC10 9。Westernblot结果证实fas基因转导株Fas EC10 9表达高水平的Fas蛋白 ;细胞生长曲线和软琼脂集落形成实验结果显示 ,与对照细胞相比 ,Fas EC10 9的群体倍增时间延长 ,克隆形成能力降低。MTT比色法表明Fas EC10 9对CDDP、VCR、5 FU的敏感性明显增加。本研究结果提示 ,转导fas基因能有效抑制体外培养的食管癌细胞的生长 ,增强癌细胞对化疗药物的敏感性
In order to study the fas gene transduction effect on esophageal cancer cells in vitro, the fas pBK vector of fas gene was constructed and transfected into esophageal cancer cell EC10 9. Westernblot analysis confirmed that the Fas gene transducing strain Fas EC109 expressed high levels of Fas protein. Cell growth curves and soft agar colony formation experiments showed that compared with the control cells, the population doubling time of Fas EC10 9 was prolonged and the colony forming ability was decreased. MTT colorimetric assay showed that the sensitivity of Fas EC10 9 to CDDP, VCR and 5 FU was significantly increased. The results of this study suggest that the transduction of fas gene can effectively inhibit the growth of esophageal cancer cells cultured in vitro and enhance the sensitivity of cancer cells to chemotherapy drugs.