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采用多导描记及免疫组化方法，观察了侧脑室和腹腔给予硝基左旋精氨酸（Ｌ－ＮＮＡ）对慢性植入电极大鼠睡眠量及中缝背核５－羟色胺（５－ＨＴ）神经元免疫阳性反应的影响。结果表明：Ｌ－ＮＮＡ显著抑制慢波睡眠和快眼动睡眠，与对照相比较，觉醒时间延长。脑室给予Ｌ－ＮＮＡ使中缝背核５－ＨＴ神经元阳性细胞增多。提示一氧化氮合成抑制所致的睡眠抑制可能与中缝背核５－ＨＴ神经元免疫阳性反应增强有关。
The effects of intracerebroventricular and intraperitoneal administration of L-arginine (L-NNA) on sleep volume and the number of 5-HT neurons in the dorsal raphe nucleus of rats were observed by multi-tracing and immunohistochemistry. The impact of metaimmune positive reaction. The results showed that: L-NNA significantly inhibited slow wave sleep and fast eye movement sleep, compared with the control, the awakening time. Cerebral ventricular administration of L-NNA increased dorsal raphe nucleus 5-HT neurons positive cells. Suggesting that inhibition of nitric oxide synthesis inhibition of sleep may be related to the 5-HT of the dorsal raphe nuclei immunoreactive positive increased.