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背景:近来骨髓基质细胞多向分化潜能的发现,特别是骨髓基质细胞向神经干细胞方向分化的成功,为干细胞移植治疗神经系统损伤提供了可能。目的:观察自体骨髓基质源干细胞对脑损伤的治疗作用。设计:以BrdU标记的自体骨髓基质源干细胞在模型动物损伤脑区和其他脑区的分布为观察对象的前瞻性研究。单位:北京军区总医院。材料:实验于1999-10/2002-02在北京军区总医院完成。取1.5～3.0岁成年家犬22只,雌雄不限,体质量(13±2)kg。随机分为假手术组6只,常规治疗组8只,骨髓基质源神经干细胞组8只。方法:对犬骨髓基质细胞进行采集、分离、体外扩增、标记和移植用细胞培养。假手术组仅做皮肤切口及颅骨锥孔,不致中脑损伤,于麻醉清醒后行正常进食。常规治疗组行中脑被盖区毁损后行三磷酸腺苷20mg,辅酶A50u,加入生理盐水中静脉滴注,2次/d,共3d。骨髓基质源神经干细胞组行中脑被盖区毁损,然后给予骨髓基质细胞源神经干细胞1mL和脑脊液1mL的混合液,注入脑室,1次/d,共3d。术后第1,7,14,28天4个时间点观察骨髓基质源干细胞在损伤脑区及其他脑区的分布情况。主要观察指标:观察骨髓基质源干细胞在损伤脑区及其它脑区的分布情况。结果:22只动物均进入结果分析。①在术后第28天:骨髓基质源神经干细胞组BrdU阳性细胞明显高于假手术组和常规治疗组[(15.4±2.4,0.5±0.5,0.5±0.5)个]。②骨髓基质源神经干细胞组术后第14天脑片BrdU阳性细胞数:中脑的伤侧明显高于对侧[(15.5±3.3,5.0±1.5)个];额叶的伤侧高于对侧[(1.6±0.6,1.5±0.7)个]。结论:由于骨髓基质源神经干细胞移植组BrdU阳性细胞数在脑损伤区的分布明显多于对侧非伤区和两个对照组的同部位。提示骨髓基质源干细胞可向损伤脑区迁移,能对脑干损伤的恢复有一定促进作用。
BACKGROUND: The recent discovery of the potential of bone marrow stromal cells to differentiate, especially the success of bone marrow stromal cells differentiating into neural stem cells, has made it possible for stem cell transplantation to treat nervous system damage. Objective: To observe the therapeutic effect of autologous bone marrow-derived stem cells on brain injury. DESIGN: Prospective study of the distribution of BrdU-labeled autologous bone marrow-derived stem cells in brain regions and other brain regions in model animals. Unit: Beijing Military Region General Hospital. MATERIALS: Experiments were performed at Beijing Military Region General Hospital from October 1999 to February 2002. Take 1.5 ~ 3.0 year-old adult dogs 22, male or female, body weight (13 ± 2) kg. Randomly divided into sham operation group 6, conventional treatment group 8, bone marrow stromal cells of neural stem cells 8. Methods: Canine bone marrow stromal cells were collected, isolated, expanded in vitro, labeled and transplanted with cells. Sham-operated group only skin incision and skull cone, will not damage the midbrain, normal anesthesia after eating normal. Routine treatment group middle cerebral artery occlusion area after the line of adenosine triphosphate 20mg, coenzyme A50u, added to the normal saline intravenous infusion, 2 times / d, a total of 3d. Bone marrow stromal cells derived from neural stem cells were impaired in the midbrain area and then mixed with 1 mL of bone marrow stromal cells derived neural stem cells and 1 mL of cerebrospinal fluid and injected into the ventricles once a day for 3 days. At the 1st, 7th, 14th and 28th postoperative days, the distribution of bone marrow stromal stem cells in the brain and other brain regions were observed. MAIN OUTCOME MEASURES: The distribution of bone marrow stromal stem cells in injured brain and other brain regions was observed. Results: All 22 animals entered the result analysis. ① On the 28th postoperative day, BrdU positive cells in bone marrow stromal cells derived from neural stem cells were significantly higher than those in sham-operation group and conventional treatment group [(15.4 ± 2.4,0.5 ± 0.5,0.5 ± 0.5)]. ② The number of BrdU positive cells in the bone marrow stromal stem cells group was significantly higher than that in the contralateral side on the 14th postoperative day [(15.5 ± 3.3, 5.0 ± 1.5)]; the injured side of the frontal lobe was higher than that of the right Side [(1.6 ± 0.6, 1.5 ± 0.7)]. Conclusion: The distribution of BrdU positive cells in brain stem cells in MSCs transplantation group is more than that in contralateral non-injured area and two control groups. Tip bone marrow stromal stem cells can migrate to the damaged brain area, can have a certain role in promoting the recovery of brain stem injury.