论文部分内容阅读
以酶法测定了血清GST活力。该法缓冲液最适pH为7.2,以60℃5分种终止酶反应最好,底物的最适浓度为0.1mmol/L。50例正常参考值(x±s,IU/L):14.66±9.7,批内与批间试验CV值均小于5％。临床测定了37例急性肝炎、14例重型肝炎、23例慢性活动性肝炎、7例肝硬化、14例原发性肝癌结果(x±s,IU/L)为66.92±19.84、95.62±16.51、46.62±12.50、43.57±10.98、23.84±7.52,与正常对照组相比有显著性差异(P<0.01或P<0.05)。GST是一较特异的肝功指标,有助于肝实质损害的预后判断。
Serum GST activity was measured enzymatically. The optimum pH of the buffer was 7.2, with 5 min at 60 ℃, the enzyme reaction was the best and the optimal substrate concentration was 0.1 mmol / L. 50 cases of normal reference value (x ± s, IU / L): 14.66 ± 9.7, intra-and inter-test CV values were less than 5%. Thirty-seven cases of acute hepatitis, 14 cases of severe hepatitis, 23 cases of chronic active hepatitis, 7 cases of cirrhosis and 14 cases of primary liver cancer (x ± s, IU / L) were 66.92 ± 19.84 and 95.62 ± 16.51, 46.62 ± 12.50, 43.57 ± 10.98 and 23.84 ± 7.52 respectively, which was significantly different from the normal control group (P <0.01 or P <0.05). GST is a more specific indicators of liver function, contribute to the prognosis of liver parenchymal damage.