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目的探讨miR-193b转染对胃癌细胞浸润、转移的影响及其作用机制。方法应用microRNA芯片检测TGF-β1处理胃癌细胞BGC823前后miRNA的差异表达谱;应用划痕实验,transwell迁移浸润及裸鼠成瘤等实验分别检测瞬时转染miR-193b抑制剂前后胃癌细胞株生物学行为的变化。结果 TGF-β1处理胃癌细胞BGC823前后miRNA的表达谱存在6个差异表达,包括3个(miR-27a,miR-29b-1和miR-194)表达上调,3个(miR-193b,miR-574-3p和miR-130b)表达下调。转染miR-193b抑制剂后,胃癌细胞株BGC823迁移、浸润和转移的能力明显增强。结论 TGF-β1可能通过下调miR-193b的表达负向调控胃癌细胞的迁移、浸润和转移。
Objective To investigate the effect of miR-193b transfection on the invasion and metastasis of gastric cancer cells and its mechanism. Methods MicroRNA microarray was used to detect the differential expression profiles of miRNAs in gastric cancer cell BGC823 treated with TGF-β1 before and after transient transfection with miR-193b inhibitor. Changes in behavior. Results There were 6 differentially expressed miRNAs in BGC823 gastric cancer cells treated with TGF-β1, including up-regulated expression of miR-27a, miR-29b-1 and miR-194, -3p and miR-130b). After transfected with miR-193b inhibitor, the ability of gastric cancer cell line BGC823 to migrate, infiltrate and metastasize was significantly enhanced. Conclusion TGF-β1 may negatively regulate the migration, invasion and metastasis of gastric cancer cells by down-regulating the expression of miR-193b.