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目的:研究苦参碱对肺间质纤维化大鼠的保护作用。方法:采用气管内灌注博来霉素(BLM)的方法建立大鼠肺纤维化模型,设正常对照组、模型组、小剂量苦参碱组(50 mg/kg)、大剂量苦参碱组(100 mg/kg)和氢化可的松组(100 mg/kg),每组18只,再各分为d 7、14和d 28组。观察各组不同时期病理切片和肺组织羟脯氨酸(HYP)浓度,测定肺组织丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH- PX)含量。结果:苦参碱组和氢化可的松组的肺泡炎和肺纤维化程度及HYP含量显著地低于模型组(P<0.05);苦参碱和氢化可的松能明显降低肺间质纤维化大鼠肺组织MDA水平,提高GSH-PX含量(P<0.01)。结论:苦参碱能减轻BLM诱导的大鼠肺纤维化,这种作用有可能通过改善肺间质纤维化大鼠体内氧化应激状态实现。
Objective: To study the protective effect of matrine on rats with pulmonary fibrosis. METHODS: Rat pulmonary fibrosis model was established by intratracheal instillation of bleomycin (BLM). The normal control group, model group, low-dose matrine group (50 mg/kg), and high-dose matrine group were established. (100 mg/kg) and hydrocortisone group (100 mg/kg), 18 in each group, each divided into d 7, 14 and d 28 groups. Observe the pathological sections and hydroxyproline (HYP) concentrations in lung tissue at different stages in each group, and measure the content of malondialdehyde (MDA) and glutathione peroxidase (GSH-PX) in lung tissue. Results: The levels of alveolitis and pulmonary fibrosis and HYP levels in the matrine group and hydrocortisone group were significantly lower than those in the model group (P<0.05); matrine and hydrocortisone significantly reduced interstitial fibrosis The level of MDA in rat lung tissue was increased and GSH-PX content was increased (P<0.01). Conclusion: Matrine can reduce BLM-induced pulmonary fibrosis in rats. This effect may be achieved by improving oxidative stress in rats with pulmonary fibrosis.