消炎利胆汤的薄层色谱法鉴别

• 作 者: 韩俊流
• 机 构: 罗田县人民医院药剂科 湖北 罗田 438600
• 出 处: 中国药师
• 发表日期: 2007年12期
• 关键词: 消炎利胆汤 薄层色谱法 鉴别

目的:采用薄层色谱法鉴别消炎利胆汤的主要原料。方法:采用大黄药材和大黄酸作对照,以石油醚甲酸乙酯-甲酸(15:5:1)为展开剂,使用硅胶G板,观察荧光色谱鉴别大黄;采用芍药苷作对照,以三氯甲烷-甲醇(4:1)为展开剂,5%香草醛硫酸溶液为显色剂,使用硅胶H板,观察日光下色谱鉴别芍药。结果:色谱中各斑点显色清晰、分离完全。结论:本法操作简便,结果准确可靠,可作为该制剂的定性分析方法。

Objective: To identify the main ingredients of Xiaoyan Lidan Decoction by TLC. METHODS: Rhubarb and rhein were used as controls, petroleum ether ethyl formate-formic acid (15:5:1) was used as developer, and silica gel G-plate was used to observe the fluorescence chromatographic identification of rhubarb; Paeoniflorin was used as the control and trichlorine was used as the control. Methane-methanol (4:1) was used as developing solvent, 5% vanillin sulfuric acid solution was used as color reagent, and silica gel H plate was used to observe the spectrum under sunlight to identify drug. Results: The spots in the chromatogram are clearly visible and completely separated. Conclusion: This method is simple, accurate and reliable, and can be used as a qualitative analysis method for the preparation.