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目的利用反相高效液相色谱法测定由不同产地光果甘草制备的甘草废渣中光甘草定的含量。方法对4种产地的光果甘草用同一种水提法制备甘草废渣;再用不同提取法制备6种总黄酮粗提物;采用SymmetryC18反相色谱柱,流动相为乙腈-水-冰醋酸(55∶44∶1),检测波长为282nm,流速为1.0mL·min-1,柱温为30℃,测定各样品中光甘草定的含量。结果光甘草定在0.09～0.45mg·mL-1内呈良好线性关系(r=0.9997),平均回收率100.2%,RSD为0.89%。6种总黄酮粗提物中光甘草定含量分别为1.31%,1.16%,1.59%,2.93%,0.98%,0.85%;4种甘草废渣中,光甘草定含量分别为0.225%,0.290%,0.211%,0.218%。结论反相高效液相色谱法简便、快速、重复性好,适用于光果甘草中光甘草定的含量测定。4种光果甘草废渣中,新疆和静的光果甘草废渣所含光甘草定含量明显高于其他产地的光果甘草废渣。此外,利用甲醇回流提取法制备光甘草定时,提取率和总黄酮出膏率都高于乙醇回流提取法和甲醇超声提取法。
OBJECTIVE To determine the content of licorice in licorice residue prepared from different sources of licorice by HPLC with reversed-phase high performance liquid chromatography. Methods Six kinds of total flavonoids crude extracts were prepared by using the same water extraction method on four kinds of licorice producing areas. Six kinds of total flavonoids crude extracts were prepared by different extraction methods. Symmetry C18 reversed-phase column was used. The mobile phase consisted of acetonitrile-water-glacial acetic acid 55:44:1), the detection wavelength was 282nm, the flow rate was 1.0mL · min-1 and the column temperature was 30 ℃. Results Guangguladine showed a good linearity (r = 0.9997) in the range of 0.09-0.45mg · mL-1 with an average recovery of 100.2% and a RSD of 0.89%. The content of light glycyrrhizin in the crude extracts of six kinds of total flavonoids were 1.31%, 1.16%, 1.59%, 2.93%, 0.98% and 0.85% respectively. The content of K. licorice in the four kinds of licorice residues was 0.225% and 0.290% 0.211%, 0.218%. Conclusion RP-HPLC is simple, rapid and reproducible. It is suitable for the determination of Guang Gancao Ding in Licorice. Among the four kinds of licorice residue, the content of licorice contained in Ganoderma lucidum residue in Xinjiang Hejing was significantly higher than that in other places. In addition, the methanol reflux extraction method for the preparation of light Glycyrrhiza uralensis, the extraction rate and total flavonoids were higher than the ethanol reflux extraction and methanol ultrasonic extraction.