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肿瘤标志物对于肺腺癌病人的临床诊断和预后具有重要意义.本研究根据临床诊断选取人肺腺癌组织和癌旁正常肺组织为研究对象,采用差速离心联合双水相法纯化组织细胞质膜,运用同位素标记相对和绝对定量技术结合高效液相色谱-串联质谱技术,鉴定出肺腺癌组织和癌旁正常肺组织的差异蛋白质41种.同癌旁正常肺组织相比,18个蛋白质在肺腺癌组织中表达上调,23个蛋白质在肺腺癌组织中表达下调.生物信息学分析发现,差异质膜蛋白质FLOT1、CAV1和ITGB1均处于蛋白质相互作用网络的重要位置,可能参与了肺腺癌相关信号转导.利用蛋白质印迹和免疫组织化学染色验证,差异蛋白质FLOT1、CAV1和ITGB1在肺腺癌织和癌旁正常肺组织的表达情况,其验证结果与蛋白质组学研究结果一致.研究结果对肺腺癌诊断标志物和肺腺癌癌变分子机理研究具有重要意义.
Tumor markers for the clinical diagnosis and prognosis of patients with lung adenocarcinoma is of great significance.In this study, based on the clinical diagnosis of human lung adenocarcinoma tissue and adjacent normal lung tissue as the research object, the differential centrifugation combined with aqueous two-phase purification of tissue cytoplasm Membrane, using isotope labeled relative and absolute quantification combined with high performance liquid chromatography-tandem mass spectrometry, identified 41 kinds of differentially expressed proteins in lung adenocarcinoma and adjacent normal lung tissue.Compared with adjacent normal lung tissue, 18 proteins Up-regulated in lung adenocarcinoma, and 23 proteins were down-regulated in lung adenocarcinoma.Analysis of bioinformatics revealed that the differential plasma membrane proteins FLOT1, CAV1 and ITGB1 are both located in the important position of the protein-protein interaction network and may participate in the lung Adenocarcinoma-related signal transduction.Western blot and immunohistochemical staining confirmed the expression of FLOT1, CAV1 and ITGB1 in lung adenocarcinoma tissues and adjacent normal lung tissues.The results of the validation were consistent with the results of proteomics research. The results of the study of lung adenocarcinoma diagnostic markers and lung cancer molecular mechanism of research is of great significance.