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自从1976年Yanagimachi等介绍精子穿透试验(SPA)以来,这一技术已广泛用于基础和临床研究。作者近7年来用这一技术监测治疗男性不育症疗法的效果,并分为以下五个方面予以介绍:(1)精索静脉曲张手术。(2)克罗米芬治疗。(3)洗精子和宫内授精(IUI)。(4)白细胞对生育力的影响。(5)TEST Yolk缓冲液对精子授孕能力的影响。作者指出精索静脉曲张行高位结扎术后,生育力的改善与SPA穿透率的改善平行。作者认为对那些仅有精子穿透率下降的精液异常患者,用高位
This technique has been used extensively in basic and clinical studies since Yanagimachi et al. Introduced the Sperm Penetration Test (SPA) in 1976. In the past seven years, the author used this technique to monitor the effect of treatment of male infertility and is divided into the following five aspects: (1) varicocele surgery. (2) Clomiphene treatment. (3) washing sperm and intrauterine insemination (IUI). (4) the impact of leukocytes on fertility. (5) Effect of TEST Yolk buffer on sperm fertility. The authors point out that after high ligation of the varicocele, improvements in fertility are paralleled by improvements in SPA penetration. The authors argue that for those who have only reduced sperm penetration in patients with abnormal semen, high use