The Role of Calcium in Mediating SlIAAs Expression in Tomato Pedicel Explants

来源 :Agricultural Sciences in China | 被引量 : 0次 | 上传用户:zhanglq
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In order to illuminate the effect of calcium on the IAA signal transduction,we studied the expression of eight SlIAA genes which were up-regulated by calcium during the abscission after short-term(1 h) and long-term(16 h) treatments with calcium,IAA and calcium inhibitors.The results indicated that calcium induced SlIAAs expression in 1 h at low concentration,EGTA affected little SlIAAs expression;it also increased IAA-induced transcript level of SlIAAs,TFP(named trifluoperazine) decreased most SlIAAs expression below the level of calcium combined with IAA-induced expression,except for SlIAA17.Long-term treatment of calcium or calcium inhibitors suppressed some SlIAAs expression,whereas TFP enhanced all SlIAAs expression.Interestingly,SlIAA29 was induced by most treatments excluding 16 h EGTA. In order to illuminate the effect of calcium on the IAA signal transduction, we studied the expression of eight SlIAA genes which were up-regulated by calcium during the abscission after short-term (1 h) and long-term (16 h) treatments with calcium, IAA and calcium inhibitors. The results indicated that calcium induced SlIAAs expression in 1 h at low concentration, EGTA affected little SlIAAs expression; it also increased IAA-induced transcript level of SlIAAs, TFP (named trifluoperazine) reduced most SlIAAs expression below the level of calcium combined with IAA-induced expression, except for SlIAA17. Long-term treatment of calcium or calcium inhibitors suppressed some SlIAAs expression, whereas TFP enhanced all S1IAAs expression.
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