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目的：通过对比ＭＤＡ－ ＮｅｏＲ 与ＭＤＡ 人乳癌细胞的生物学特征，阐明原核标志基因ＮｅｏＲ 的长期稳定整合与表达是否对乳癌细胞原有的生物学特性产生影响。方法：对已建立且证实表达ＮｅｏＲ 基因＞ ６ 个月的７ 株ＭＤＡ－ＮｅｏＲ 细胞系进行Ｇ４１８ 抗性、表面抗原、细胞倍增时间、细胞周期、肿瘤细胞集落形成及裸鼠体内成瘤能力的检测。结果：ＭＤＡ－ ＮｅｏＲ 细胞对高浓度的Ｇ４１８ 仍产生抗性并表达Ｎｅｏ Ｒ 基因；其细胞倍增时间、Ｓ 期分数、肿瘤细胞体外集落形成率与ＭＤＡ 无明显区别（ Ｐ＞ ０－０５） ；角蛋白ＣＫ１９ 与表面膜抗原ＥＭＡ 的表达在两组是等同的；并且ＭＤＡ－ ＮｅｏＲ ７ 细胞系在裸鼠体内的成瘤能力与ＭＤＡ 细胞也是相似的。结论：外源标志基因ＮｅｏＲ 的长期稳定整合与表达对ＭＤＡ 人乳癌细胞的原有生物学特性无明显影响
Objective: To compare the biological characteristics of MDA-NeoR and MDA human breast cancer cells to elucidate whether the long-term stable integration and expression of prokaryotic marker gene NeoR affects the original biological characteristics of breast cancer cells. METHODS: G418 resistance, surface antigen, cell doubling time, cell cycle, tumor cell colony formation, and tumorigenicity in nude mice were determined in 7 MDA-NeoR cell lines that had been established and confirmed to express NeoR genes for > 6 months. . RESULTS: MDA-NeoR cells were still resistant to high concentrations of G418 and expressed the Neo R gene; their cell doubling time, S-phase fraction, and tumor cell formation in vitro were not significantly different from those of MDA (P> 0-05); The expression of protein CK19 and surface membrane antigen EMA is equivalent in both groups; and the tumorigenicity of MDA-NeoR7 cell line in nude mice is similar to that of MDA cells. Conclusion: The long-term stable integration and expression of exogenous marker gene NeoR has no significant effect on the original biological characteristics of MDA human breast cancer cells.