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采用聚丙烯酰胺垂直平板电泳技术对披碱草和野大麦及其杂种 F1和 BC1过氧化物酶(POD)同工酶做了比较分析。结果表明 ,亲本披碱草和野大麦的 POD同工酶谱可明显地分成A、B两区 ,有 4条相同位点的基带 ,从酶蛋白分子水平验证出两亲本有较近的亲缘关系 ;杂种 F1的 POD酶谱主要表现为双亲酶带的互补类型 ,正交 (野大麦×披碱草 ) F1和反交 (披碱草×野大麦 ) F1的酶谱基本相同 ,在个别位点存在酶带活性强弱的差别 ;野大麦×披碱草×野大麦 (BC1)和披碱草×野大麦×野大麦 (BC1)的酶谱基本相同 ,但也存在一定的差别 ,BC1的酶谱明显偏向轮回亲本野大麦 ,特别是野大麦×披碱草×野大麦 ,说明通过回交使野大麦的某些性状在 BC1代中进一步加强 ;POD同工酶具有多态性 ,可作为遗传标记用于杂种的鉴定和回交后代目标性状植株的检测。
Polyacrylamide vertical plate electrophoresis technique was used to analyze the isozymes of Elymus dahuricus and Hordeum brevisubulatum and their hybrid F1 and BC1 peroxidase (POD). The results showed that POD isozymes of Elymus dahuricus and Hordeum brevisubulatum were clearly divided into A and B regions and there were 4 bases in the same locus. ; The POD zymogram of hybrid F1 mainly showed the complementary type of the parental enzyme band. The zymogram of F1 between Orthogonal (Wild barley × Elymus carassius) F1 and the reciprocal cross (Elymus × Barley) was basically the same, There were differences in the activity of enzyme bands; Enzymes of wild barley × Elymus × barley (BC1) and Elymus × wild barley × wild barley (BC1) were similar but there were some differences The spectrum was obviously biased toward the reincarnated parent barley, especially wild barley × Elymus × barley, which indicated that some traits of wild barley were further strengthened in BC1 by backcrossing; POD isozymes were polymorphic and could be used as genetic Markers are used for the identification of hybrids and the detection of target traits in backcross progeny.