论文部分内容阅读
目的通过观察慢性应激对小鼠学习记忆能力以及脑组织神经肽Y(NPY)含量的变化,探讨慢性应激对学习记忆和神经肽Y的影响。方法采用束缚制动的方法建立慢性心理应激模型,应激结束后进行Morris水迷宫实验检测小鼠应激状态下的学习记忆能力,并采用放射免疫方法检测小鼠脑组织中NPY的含量。结果应激组小鼠在Morris水迷宫中寻找平台的潜伏期较对照组显著延长(P<0.01),在平台所在象限的游泳时间比例显著小于对照组(P<0.01)。与对照组相比,应激组小鼠脑组织NPY的表达显著增加(P<0.01)。结论慢性应激可引起机体的学习记忆能力的改变,主要表现为注意力不集中、记忆力减退;慢性应激可导致小鼠脑组织NPY的高表达,且NPY与心理应激引起小鼠学习记忆能力的改变相关,提示NPY可能作为应激分子参与机体的心理应激过程。
Objective To investigate the effect of chronic stress on learning and memory and neuropeptide Y in mice by observing the changes of learning and memory ability and neuropeptide Y (NPY) levels in mice. Methods The chronic psychological stress model was established by restraint braking method. Morris water maze test was used to detect the learning and memory ability of mice under stress and the radioimmunoassay was used to detect the content of NPY in mice brain. Results The latent period of looking for platform in the Morris water maze was significantly longer than that in the control group (P <0.01). The swimming time in the platform where the platform was located was significantly smaller than that in the control group (P <0.01). Compared with the control group, the expression of NPY in stress group increased significantly (P <0.01). Conclusion Chronic stress can cause the change of learning and memory in the body, which is mainly due to lack of concentration and memory loss. Chronic stress can lead to the high expression of NPY in mice brain, and NPY and psychological stress cause mice learning and memory Related to changes in ability, suggesting that NPY may participate as a stressor in the body’s psychological stress process.