甘蓝×白菜种间花粉-柱头相互作用的研究

• 作 者: 巩振辉 何玉科 王飞 李华君
• 机 构: 西北农业大学园艺系,西北农业大学园艺系,西北农业大学园艺系,西北农业大学园艺系 陕西杨陵·712100,陕西杨陵·712100,陕西杨陵·712100,陕西杨陵·712100
• 出 处: 西北农林科技大学学报(自然科学版)
• 发表日期: 1992年03期
• 关键词: 柱头 甘蓝 花粉 白菜 远缘杂交 花粉管 杂交不育 相互作用 白菜品种 母本

以甘蓝为母本与白菜杂交,授粉后采不同时期的子房用苯胺兰法染色,在荧光显微镜下观察花粉在柱头上粘合、萌发及花粉管在柱头表面和花柱中的生长情况。试验结果表明:甘蓝×白菜杂交的主要障碍在于白菜萌发的花粉管难以伸进柱头,并与柱头表面发生严重的胼胝质反应,使花粉管扭曲,尖端膨大,难以生长。

The cabbage was taken as the female parent and the Chinese cabbage was crossed. After pollination, the ovaries were stained with the method of fonnyl blue at different stages. The growth of the pollen on the stigma was observed by fluorescence microscopy. The results showed that the main obstacle to the hybridization of cabbage × cabbage was that it was difficult for the pollen tube of cabbage to penetrate into the stigma and serious callose reaction with the stigma surface, distorting the pollen tube and swelling the tip of the pollen tube.