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目的 建立基于16SrRNA基因的PCR-Dot blot细菌学检测方法,用于慢性前列腺炎患者EPS的细菌学检查,以指导临床诊断和治疗。方法 建立PCR-Dot blot方法,并对实验组58例标本和对照组30例标本进行检测。结果 经过优化的PCR方法检测细菌的敏感性达到了103/mL。实验组标本PCR检测阳性率46.6%(27/58),其中包括培养法阳性的4例标本占实验组总数的7.0%(4/58)。对照组标本PCR检测阳性率为10%(3/30)。经过杂交检测实验组标本中20例为G-菌,7例为G+细菌,对照组3例PCR阳性标本都为G+细菌。结论 基于16SrRNA基因的PCR-Dot blot方法具有快速、简便、稳定、不受干扰等优点,可用于检测慢性前列腺炎患者的EPS。
Objective To establish a PCR-Dot blot bacteriological assay based on 16S rRNA gene for the bacteriological examination of EPS in patients with chronic prostatitis to guide clinical diagnosis and treatment. Methods PCR-Dot blot method was established, and the experimental group of 58 specimens and control group of 30 specimens were detected. Results The sensitivity of the optimized PCR method to detect bacteria reached 103 / mL. The positive rate of PCR test in experimental group was 46.6% (27/58), including 4 cases positive in culture method accounting for 7.0% (4/58) of the total in experimental group. The positive rate of PCR test in control group was 10% (3/30). After hybridization test, 20 cases were G-bacteria in the experimental group and 7 cases were G + bacteria in the test group, and 3 cases in the control group were G + bacteria. Conclusion The PCR-Dot blot method based on 16S rRNA gene has the advantages of fast, simple, stable, and free from interference. It can be used to detect EPS in patients with chronic prostatitis.