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ADP被认为是动脉血栓形成的一个重要因素。在体外,ADP可诱发血小板内Ca2+浓度增高,为了探讨其机制及在临床诊断中的价值,我们应用流式细胞仪[1]和激光扫描共聚焦显微镜[2]观察负载ca2+荧光深外Fluo-3/AM的血小板在ADP作用下细胞内Ca2+变化。材料和方法1材料1.1试剂1.1.I
ADP is considered as an important factor in arterial thrombosis. In vitro, ADP induced platelet Ca2 + concentration increased, in order to explore the mechanism and the value in the clinical diagnosis, we use flow cytometry [1] and laser scanning confocal microscope [2] observed loading ca2 fluorescence deep Fluo- 3 / AM platelets under the action of ADP intracellular Ca2 + changes. Materials and Methods 1 Materials 1.1 Reagents 1.1. I