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发卡式核酶的一级结构为RNA分子,切割活性所需的最小长度为50个核苷酸,其立体结构由四个螺旋区及数个环状区域组成,核酶结构中有15个核苷酸是必需的,它们的改变对核酶活性和立体结构都会产生显著的影响,本文综述了发卡式核酶结构与功能的研究进展,并讨论了其作用机理和应用原则。
The primary structure of the hairpin ribozyme is an RNA molecule, the minimum length required for cleavage activity is 50 nucleotides, the three-dimensional structure consists of four helix regions and several ring-shaped regions, and 15 nuclei in the ribozyme structure Glyoxal is essential, and their changes have a significant impact on the activity of ribozymes and three-dimensional structure. This review summarizes the research progress of the structure and function of hairpin ribozymes, and discusses its mechanism of action and application principles.