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目的探讨Th17细胞相关细胞因子IL-17、IL-6,IL-1β等在原发性肾小球疾病的表达水平及其意义,以期为诊治原发性肾小球疾病找到新的治疗靶点提供实验室依据。方法将研究对象分为实验组和对照组,实验组为原发性肾小球疾病患儿30例,取其外周血;并收集10例健康体检儿童外周血作为正常对照组。通过荧光定量PCR检测对IL-17mRNA在肾小球疾病患儿及健康儿童外周血中的表达进行分析;酶联免疫吸附试验(ELISA)分别检测血浆中IL-17、IL-1β以及IL-6的表达。结果 (1)原发性肾小球疾病儿童IL-17mRNA表达水平显著高于正常对照组(P<0.05)。(2)肾组织内,实验组患儿血浆中IL-17、IL-1β以及IL-6的表达明显高于正常对照组(P<0.05)。结论 Th17细胞相关炎性因子在原发性肾小球疾病的发病机制中起着重要作用,特异性针对Th17细胞相关炎性因子的治疗可能是治疗原发性肾小球疾病的新靶点。
Objective To investigate the expression and significance of Th17 cell-associated cytokines IL-17, IL-6 and IL-1β in primary glomerular diseases and to find new therapeutic targets for the diagnosis and treatment of primary glomerular diseases Provide laboratory basis. Methods The subjects were divided into experimental group and control group, the experimental group was 30 cases of primary glomerular disease in children, and the peripheral blood was collected. 10 healthy children were collected as normal control group. The expression of IL-17 mRNA in peripheral blood of children with glomerular disease and healthy children was analyzed by real-time PCR, and the levels of IL-17, IL-1β and IL-6 in plasma were detected by enzyme linked immunosorbent assay (ELISA) expression. Results (1) The expression of IL-17mRNA in children with primary glomerular disease was significantly higher than that in the normal control group (P <0.05). (2) The expression of IL-17, IL-1β and IL-6 in renal tissue of experimental group was significantly higher than that of normal control group (P <0.05). Conclusions Th17 cell-associated inflammatory cytokines play an important role in the pathogenesis of primary glomerular diseases. The treatment of Th17 cell-associated inflammatory cytokines may be a new target for the treatment of primary glomerular diseases.