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为了鉴定水稻对稻曲病的抗病基因,利用157个家系组成的大关稻(japonica)/IR28(indica)重组自交系(Recombinant inbred lines,RIL)群体,采用人工接种方法 ,以发病病情指数作为表型值。2012和2013年,接种鉴定亲本及RILs对水稻稻曲病致病菌株Pi-1的抗性。利用QTL Cartographer分析软件,对水稻抗Pi-1菌株基因进行检测分析。两年共检测到7个QTL,分别为位于第2、7、8、11和12染色体上的q Fsr2a、q Fsr2b、q Fsr7、q Fsr8a、q Fsr8b、q Fsr11、q Fsr12,单个位点的贡献率介于8.5%~17.2%之间。其中,2012年检测到q Fsr2a、q Fsr2b、q Fsr8a、q Fsr11等4个位点;2013年检测到q Fsr7、q Fsr8b、q Fsr11、q Fsr12等4个位点。q Fsr11在两年中均被检测到,对性状的解释率为13.5%和17.2%,作用效应使病情指数下降9.9%和14.3%,提高了抗病性。根据抗性位点加性效应方向,q Fsr2a、q Fsr8a、q Fsr8b、q Fsr11和q Fsr12等位点是来自于亲本IR28的增效等位基因,而位点q Fsr2b和q Fsr7的抗性效应方向相反,是来自于大关稻。稳定遗传的抗病位点q Fsr11及其紧密连锁的分子标记可以在分子标记辅助选择育种中得以应用。
In order to identify the resistance genes to rice false smut in rice, 157 populations of recombinant inbred lines (RIL) from japonica / IR28 (indica) were inoculated by artificial inoculation Index as phenotype value. In 2012 and 2013, the parents and RILs were tested for their resistance to the rice pathogenic strain Pi-1. The QTL Cartographer analysis software was used to detect and analyze the rice anti-Pi-1 gene. Seven QTLs were detected in two years, which were q Fsr2a, q Fsr2b, q Fsr7, q Fsr8a, q Fsr8b, q Fsr11, q Fsr12 on chromosomes 2, 7, 8, 11 and 12 respectively The contribution rate is between 8.5% ~ 17.2%. Among them, four loci such as q Fsr2a, q Fsr2b, q Fsr8a and q Fsr11 were detected in 2012, and four loci qfsr7, q Fsr8b, q Fsr11 and q Fsr12 were detected in 2013; q Fsr11 was detected in both years, with explanations of traits 13.5% and 17.2%, and the effect index decreased disease index by 9.9% and 14.3%, improving disease resistance. Based on the additive effect of the resistance loci, the alleles qFsr2a, qFsr8a, qFsr8b, qFsr11 and qFsr12 were all alleles derived from the parental IR28, whereas the loci qFsr2b and qFsr7 were resistant The opposite direction of the effect comes from the big paddy rice. Stable genetic resistance locus q Fsr11 and its closely linked molecular markers can be used in molecular marker-assisted selection breeding.