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目的:观察中药制剂川芎嗪对ＳＤ大鼠视网膜缺血后细胞凋亡的影响。方法:采用前房灌注生理盐水的方法做成视网膜缺血模型,治疗组每天腹腔注射川芎嗪１次(５０ ｍｇ/ｋｇ)。Ｔｕｎｅｌ法检测各组视网膜凋亡细胞,结果:缺血组凋亡细胞明显高于川芎嗪治疗组,同一时间段各组之间相比差异均有显著性。结论:川芎嗪可减少缺血后视网膜细胞的凋亡,具有明显的保护作用。
OBJECTIVE: To observe the effect of traditional Chinese medicine preparation ligustrazine on apoptosis after retinal ischemia in SD rats. Methods: Retinal ischemia model was made by infusing normal saline into the anterior chamber. The treatment group received intraperitoneal injection of tetramethylpyrazine once daily (50 mg/kg). Tunel assay was used to detect apoptotic cells in each group. Results: The apoptotic cells in the ischemic group were significantly higher than those in the tetramethylpyrazine treatment group. There was a significant difference between the groups at the same time. Conclusion: Tetramethylpyrazine can reduce the apoptosis of retinal cells after ischemia and has obvious protective effect.