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为防止伴性遗传病的发生,人们竭力寻求一些简便有效的方法分离含有x-或Y-染色体的精子进行人工授精或体外受精/胚胎移植。本文介绍一种较简便的梯度密度离心的方法。 将正常的精液标本分3组进行试验:对照组(不经处理);单纯用Percoll液处理(2组);联合应用Percoll液和NycoPrep液处理(3组)。Pereoll液用10
In order to prevent the occurrence of sexually transmitted diseases, people are trying to find some simple and effective ways to separate sperm containing x- or Y-chromosomes for artificial insemination or IVF / ET. This article presents a simpler method of gradient density centrifugation. Normal semen samples were divided into 3 groups: Control group (untreated); Percoll alone (2 groups); Percoll plus NycoPrep (3 groups). Pereoll fluid with 10