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小麦矮秆突变体DC20是经高能混合粒子场处理得到的,表现为赤霉素敏感型。本文采用实时荧光定量PCR技术,研究该突变体矮秆性状与GA合成及信号转导途径相关基因表达的关系。共检测编码GA合成途径关键酶和编码信号转导途径作用因子的10个关键基因。其中,编码内-贝壳烯杉氧化酶(KO)的GA3基因和编码正调控因子基因GAMYB的表达量极显著下调,下调倍数分别为20.0、3.1;2个编码负调控因子基因GAI和SPY显著上调,上调倍数分别为2.3、1.3。外源GA3(赤霉酸)处理能够抑制编码负调控因子基因GAI、SPY和RGA的转录,而GA合成关键酶基因GA3及正调控因子基因GAMYB转录水平显著升高表明DC20矮秆基因对GA相关途径的受外源GA3的调节。
Wheat dwarf mutant DC20 is obtained by high-energy mixed particle field processing, showing gibberellin-sensitive. In this paper, real-time fluorescent quantitative PCR was used to study the relationship between the dwarf traits of the mutant and the gene expression of GA synthesis and signal transduction pathways. A total of 10 key genes encoding the key enzymes of the GA synthesis pathway and the factors that encode the signal transduction pathway were examined. Among them, GA3 gene encoding the inner-shell serous cedar oxidase (KO) and the gene encoding the positive regulator gene GAMYB were significantly down-regulated by 20.0 and 3.1, respectively; GAI and SPY genes encoding two negative regulators were significantly up-regulated , Up multiples were 2.3,1.3. Exogenous GA3 treatment inhibited the transcription of the genes encoding GA1, SPY and RGA, while GA3, a key gene of GA synthesis, and GAMYB, a positive regulator of transcription, were significantly increased, indicating that the DC20 dwarf gene was associated with GA Pathway regulated by exogenous GA3.