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为研究辐照实蝇的鉴别方法,采用10~160Gy辐照桔小实蝇卵和不同日龄幼虫后,分别测定单条幼虫体内的酚氧化酶(PPO)、乙酰胆碱酯酶(ACh E)、超氧化物歧化酶(SOD)的OD值,并观察虫体黑化情况。结果表明:80 Gy以上剂量辐照桔小实蝇卵、不同日龄幼虫后,能显著降低7日龄幼虫体内PPO、SOD的OD值;80 Gy以上剂量辐照桔小实蝇24 h卵和1日龄幼虫后,能显著降低其7日龄幼虫体内ACh E的OD值;因此通过测定SOD、PPP、ACh E酶活性变化,可以作为判定桔小实蝇是否经过检疫辐照处理的依据。最低以40 Gy辐照桔小实蝇卵、1日龄、2日龄幼虫后,其7日龄幼虫虫体不能黑化,而对照虫体黑化,因此观察虫体黑化亦可作为判定桔小实蝇辐照与否的依据。
In order to study the identification method of irradiated fruit flies, the contents of phenoloxidase (PPO), acetylcholinesterase (ACh E) and ultrasonication of single larvae were measured after irradiated with 10 ~ 160Gy eggs and different day-old larvae Oxidative dismutase (SOD) OD values, and observe the black body parasites. The results showed that the OD values of PPO and SOD in 7-day-old larvae were significantly lower than those of adult larvae at 80 Gy irradiation for 24 h, 1-day-old larvae could significantly reduce the ACh E OD value of 7-day-old larvae. Therefore, the determination of ACh E activity by SOD, PPP and ACh E could be used as the basis for judging the occurrence of ACh E. After the 4th instar larvae were exposed to 40 Gy, the larvae of 7-day-old larvae could not be blackened and the black larvae of the control animals were blackened The basis of irradiation or not on orange fruit fly.