叶酸及细胞穿膜肽修饰的纳米脂质载体穿透体外肿瘤球模型研究（英文）

• 作 者: 庄鸿蒙 孟婷婷 高玮 袁兰 齐宪荣
• 机 构: 北京大学医学部药学院 分子药剂学和新释药系统北京重点实验室,北京大学医学部药学院药剂学系,北京大学医学部药学院 药剂学系,北京大学医学部医学分析中心,
• 出 处: Journal of Chinese Pharmaceutical Sciences
• 发表日期: 2015年10期
• 关键词: 穿膜肽 纳米 纳米脂质载体 肿瘤球 球模型 堆积法 肿瘤 脂质 叶酸 细胞穿膜肽

纳米递药系统的表面修饰作用会对载体的穿透能力产生影响。本研究通过使用叶酸配体和细胞穿膜肽分别修饰的纳米脂质载体,在堆积法建立的肿瘤球模型上实时分析细胞靶向和细胞穿透作用对于纳米脂质载体的肿瘤穿透能力的影响。研究结果表明细胞穿透肽修饰的纳米脂质载体不论是从穿透深度还是强度上,在穿透早期都具备更好的穿透肿瘤球能力。

The surface modification of the nano-delivery system can have an impact on the ability of the carrier to penetrate. In this study, the cell-targeting and cell-penetrating effects were evaluated in real-time on a tumor-spheroid model established by the hematocrit method using a nanolipid vector modified with folate ligand and cell-penetrating peptide, respectively, for the tumor-penetrating ability of the nanolipid vector influences. The results show that cell-penetrating peptide-modified nano-lipid carriers have better capability of penetrating tumor spheres, both in penetration depth and in intensity, during the early stage of penetration.