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本文作者对正常人外周血淋巴细胞的大量分离和激活条件以及淋巴因子的分离提纯进行了研究,并获得一定量的淋巴因子(不含正常血清蛋白成分或外源凝集素)。通过琼脂糖电泳、连续浓度梯度聚丙烯酰胺凝胶电泳、等电聚焦和交联葡聚糖凝胶过滤证明淋巴因子是一组在泳动速度、分子量、等电点等方面都极不均一的蛋白质。用淋巴因子制备的多价抗血清进行线状免疫电泳证明:正常人少量全血经PHA或CbnA激活6～24小时,在去血球的上层液中,在pH8.6向正极和向负极泳动的某些淋巴因子生成量均较对照组明显增多,这种差别有可能在一定程度上反映T淋巴细胞的功能。
The authors studied the isolation and activation conditions of peripheral blood lymphocytes in normal people and the purification of lymphokines and obtained a certain amount of lymphokines (without normal serum protein components or lectins). Agarose gel electrophoresis, continuous concentration gradient polyacrylamide gel electrophoresis, isoelectric focusing and cross-linked Sephadex gel filtration proved that lymphokines are extremely inhomogeneous in terms of swimming speed, molecular weight, isoelectric point, etc. protein. The linear immunoelectrophoresis of multivalent antiserum prepared with lymphokine showed that a small amount of normal human blood is activated by PHA or CbnA for 6-24 hours. In the supernatant of the dehemosphere, it migrates to the positive and negative electrodes at pH 8.6. The production of certain lymphokines was significantly increased compared with the control group, and this difference may reflect the function of T lymphocytes to some extent.