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目的:对金荞麦MYB转录因子基因FdMYBP1进行克隆和序列特征分析。方法:采用RACE结合cDNA文库筛选的方法,从金荞麦cDNA文库中克隆MYBP1基因(FdMYBP1);生物信息学分析,Southern杂交。结果:克隆到1个MYBP1转录因子基因(FdMYBP1),通过Southern杂交分析,推测FdMYBP1基因是1~2个拷贝基因;FdMYBP1基因编码1个长256个氨基酸的蛋白质,在N端存在1个保守结构域,属于MYB转录因子家族。结论:首次从金荞麦中克隆到转录因子基因FdMYBP1,它具有MYB同源基因的典型特征,可能在金荞麦类黄酮代谢途径中起作用。
OBJECTIVE: To clone and sequence characterize the MYB transcription factor FdMYBP1 from Fagopyrum californica. Methods: MYBP1 gene (FdMYBP1) was cloned from the cDNA library of F buckle by RACE combined with cDNA library screening; bioinformatics analysis and Southern blotting. Results: One MYBP1 transcription factor gene (FdMYBP1) was cloned by Southern blot analysis. FdMYBP1 gene was predicted to be one to two copies of the gene. FdMYBP1 gene encoded a protein of 256 amino acids with a conserved structure at the N-terminus Domain, belonging to the MYB transcription factor family. Conclusion: The transcription factor FdMYBP1 was cloned from Fagopyrum tataricum for the first time. It has the typical characteristics of MYB homologous genes and may play a role in the flavonoid metabolism pathway of Fagopyrum.